Use Blast for the three species of Leishmania in order to take only the common sequences in fasta file
I am using Blastp for the three species of Leishmania (Linfantum, Major, Braziliensis) in order to take only the common sequences in fasta file . I created db of Major and Braziliensis in order to have the Linfantum vs both of them and then i want to create a new fasta of linfantum (linfantum.fasta) only with the sequences which are common.
Do you know how to do this with Blastp ?
Do i have to use a specific evalue parameter ?
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dzisis1986 •
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dzisis1986 •
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It may be better to do this using CD-HIT.
If you're just interested in finding core orthologs between genomes, you could also try ROARY.