small RNA sequence data
Hi,
We used NEBNext Small RNA Library Prep Set for Illumina for small RNA sequencing experiment (and size selected). In case, if the dsRNA substrate is generated from the products of convergent transcription and the transcript itself does not have a hairpin structure, is it possible conclude from the sequence data whether the small RNAs are indeed dicer processed, but not a degraded RNA?
Thank You. Any help is appreciated.
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krishnendu •
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