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Plylogenetic tree construction

I have amino acid sequences of about 900 genes from 20 species and I want to construct a phylogenetic tree from it. I read that these are to aligned first by MUSCLE and then to create a tree. I want to know what would be best tool for the construction of such a big tree.

Thanks

sequence alignment muscle tree

3 answers

RAxML - https://sco.h-its.org/exelixis/web/software/raxml/index.html

Be sure to view and your alignment so you can see errors.

You can use MUSCLE alignment for a maximum of 500 sequences or a maximum file size of 1 MB. The choice of tool for phylogenetic tree construction also depends on kind of tree exactly you want to look at from it.

Following two works good:

MEGA

TreeView

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