Question

PINDEL and somatic mutations

0

Entering edit mode

6.5 years ago

Leandro de Mattos ▴ 90

For illumina I need to Recalibrated bases and Indelrealigned. I would like to known if Is necessary Recalibrated bases and Indelrealigned (GATK software) of bam (IonTorrent) for further Pindel Analysis?? Thanks, Leandro

pindel indelrealigned GATK • 1.5k views

ADD COMMENT • link updated 5.8 years ago by Kevin Blighe 87k • written 6.5 years ago by Leandro de Mattos ▴ 90

score 0 · Answer 1 · 2018-07-17

0

Entering edit mode

5.8 years ago

Kevin Blighe 87k

Oi Leandro,

Não, não e necessário. Você só tem que mapear os seus reads à genoma da referencia, como hg38.fasta e com, per exemplo, bwa mem (se os seus reads tem um comprimento de >70bp).

Obrigado / Abraços, Kevin

Translation: [Hey Leandro, No, it's not necessary..You just have to align your reads to the reference genome, like hg38.fasta, and with, for example, bwa mem (if the reads have length >70bp). Thanks / Hugs, Kevin]

ADD COMMENT • link 5.8 years ago by Kevin Blighe 87k

1

Entering edit mode

Eu que agradeço Kevin. Abs

Em terça-feira, 17 de julho de 2018, Kevin Blighe on Biostar < mailer@biostars.org> escreveu:

ADD REPLY • link 5.8 years ago by Leandro de Mattos ▴ 90