nucleotide frequency of each gene at certain position
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The information you have provided is not so helpful for someone to answer / give you suggestion.
What kinda of data you have? BAM? Simple fasta? Do you have positions in BED format? And why do you want to do that?
Providing few details in the main thread will always be helpful for you and others.
Don't forget to follow up on your previous threads, multiple are without accepted answer.
If an answer was helpful you should upvote it, if the answer resolved your question you should mark it as accepted.