Hello everyone,

I look to setup a single cell-RNAseq experiment with the C1 fluidigm platform. In the protocol, the advice to use Spike-in RNA for normalization (Ambion AM1780). So I look on the website of thermofisher, but I cannot find any sequences of these Spike-in. I made some research, and peoples are used to modify the index file to align these control, but without the sequence I don't know how to do that.

Does anybody used these spike-in for experiments, and have a solution to implement the index file to align reads?

Thanks a lot,

nicolas

Ps: I sent an email to the support, currently waiting for an answer.