Differential Expressed Genes by FPKM expression matrix
Hello everybody
I have a FPKM RNA-seq expression matrix. which R package is good to calculate differential expressed genes?
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morteza.kouhsar •
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morteza.kouhsar •
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You can use ballgown provided you have data in ballgown required format. edgeR and DEseq2 needs raw counts.
How many replicates do you have?
The data contains 457 samples and about 43000 RNAs.
i want to know how to make RNA-seq expression matrix by FPKM
1) Please leave the answer field for answers. 2) Some effort is appreciated if you ask a question, 3) please read available resources https://www.bioconductor.org/packages/devel/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html
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