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Transcriptomic features of peaks at mature mRNA level

Hi,

I have a list of peaks that I want to map into genome and get different genomic features. I would like to see whether the peaks are mapped into:

5'CAP --> 5'UTR --> CDS --> STOP CODON --> 3'UTR

Are there any programs or ways to do this?

I have already looked at ChIPpeakAnno and ChIPseeker (both in Bioconductor). Both programs map peaks into
Promoter, 5'UTR, 3'UTR, Exons, immediateDownstream and Introns.

Many thanks, Ezhil

chip-seq genome

What kind of peaks are this? You talk about genomic regions but provide transcriptomic features you want to map to. Please specify what your goal is.

Sorry, it should be 'Transcriptomic features' not the genomic features. These are the peaks obtained after m6A modification. My goal is to see the distribution of peaks on those transciptomic features.

1 answer

You can try bedtools intersect. Given a set of peaks (in bed format for instance) and an annotation file, this can give you the features covered by the peaks.

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