Hello

I have decided to use HYPHY software in order to find positive selection sites in a negative sense RNA genome virus. My input file is a multiple aligned sequences from CLUSTAL X software of which I have saved in nxs format. based on tutorials, I have done the following steps: New Analysis> nxs file> standard analysis> positive selection> quick selection detection> universal> new analysis> nxs file (again as a data file)> custom model> tree file in ph format (saved in CLUSTAL X software) .... but here comes the error with two note:

1 : Build Tree givenTree from string treeString

2 : ExecuteAFile from file "qndhelper1.ibf" using basepath C:\Program Files (x86)\HyPhy 2.2.3\TemplateBatchFiles.

1. what should I do now to continue?
2. am I choosing the right steps for viral RNA genome selection detection?

Thanks !