Glad that you got it solved.
Best of luck!
Hi, I have the following problem:
I have in my hands the file 'merged_abundance_table.txt' and I want to create a heatmap of relative abundance for Genus. How should I rearrange the comand below?
linux@ubuntu:~$ grep -E "(s__)|(^ID)" merged_abundance_table.txt | grep -v "t__" | sed 's/^.*s__//g' > merged_abundance_table_species.txt
Can you help me please?
Thanks
HI Kevin, thanks for the reply. But I had already solved the question. Knowing that it had multiple '.fna', I was able to resolve the issue with the following command sequence:
linux@ubuntu:~$ for f in *.fna; do metaphlan2.py $f --input_type fasta --nproc 4 --tax_lev g > ${f%.fasta.gz}_profile.txt; done
linux@ubuntu:~$ merge_metaphlan_tables.py *_profile.txt > merged_abundance_table.txt
linux@ubuntu:~$ grep -E "(g__)|(^ID)" merged_abundance_table.txt | grep -v "t__" | sed 's/^.*g__//g' > merged_abundance_table_genus.txt
linux@ubuntu:~$ hclust2.py -i merged_abundance_table_genus.txt -o abundance_heatmap_genus.png --ftop 25 --f_dist_f braycurtis --s_dist_f braycurtis --cell_aspect_ratio 0.5 -l --flabel_size 6 --slabel_size 6 --max_flabel_len 100 --max_slabel_len 100 --minv 0.1 --dpi 300
Thank you very much anyway !! :)
Glad that you got it solved.
Best of luck!
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Can you show the first few lines of merged_abundance_table.txt ? ..also, an example of the format you want to produce would help a great deal.
Thanks! Kevin