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How to construct an species phylogenetic tree?

Hi,

how do you proceed to make an species phylogenetic tree? I have the NCBI accession numbers of the species. I know that I should make an 16S RNA phylogenetic tree with raxML or FastTree (after alignment with clustalo, MUSCLE or similiar tools), but is that actually correct? Can you please help me here?

Thanks!

genome gene sequence alignment

Why don't you try making the phylogenetic tree using clustal omega?

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