normalization constant in GFOLD

Question

[ashish]

I am trying GFOLD on an unreplicated RNA-seq data sets. I have two queries regarding it.

1. what is the normalization constant and what does the values signify?
2. In one of the datasets I recieved a warning saying the median of gene read count is zero. What does this mean?

-VL1 Calculating normalization constant ...
-VL1 WARNING: The median of gene read count is zero. The normalization method proposed by DESeq cannot be used. Use total read count to do normalization.
-VL1 Normalization constant is: 
     mouse_15    6.04383e+07    2.61471
     mouse_17    2.31147e+07    1
-VL1 Calculate RPKM ...