clone based sequencing

Is there anybody could tell me a little bit about the steps of clone-based sequencing?

Thanks

sequencing

• 1,117 views • link

written 8.9 years ago by mm521 •

This sort of information is easily found by a web search or in a molecular biology textbook.

• 0 views • link

written 8.9 years ago by GenoMax

0 answers

No answers yet.

More posts like this

Searching for RNA-seq melanoma samples in TCGA or cBioPortal
written 4.1 years ago by AlexStar •

I'm a junior bioinformatician, and I know TCGA and cBioPortal are among the most important databases. I'm trying to find as much data as possible …
% inhibition formula
written 7.6 years ago by saqib_jalil2002 •

hi dear frineds i am little bit confuse in % inhibition formula. can any one tell me how we calculate % inhibition of unknow compounds …
Forum: Up-to-date RNA-Seq Analysis Training/Courses/Papers
written 7.9 years ago by kullboys •

Hello everyone I am a PhD student with biology background. I recently inherit a RNA Sequencing project from another PhD student in my lab. We …
how to name a gene
written 8.9 years ago by mm521 •

I recently use flybase.org to learn bioinformatics. I just found some gene has no name or no CG name. Is there anyone could tell what …
what is scaffold
written 8.9 years ago by mm521 •

In shotgun sequencing, there is a term called scaffold and it seems that this component is made of contig. What I think here is that …
Forum: shotgun sequencing and clone-based sequencing
written 8.9 years ago by mm521 •

For WGS sequencing, the DNA was sheared first and then cloned into a vector, finally assembly contig and PCR gaps. 1. I found clone is …
Please help me with the fate of multimapping reads in the RNA-seq context
written 9.2 years ago by seta

Hi all, I read a sentence in a book about RNA-seq analysis, it says "Some aligners are able to distribute multimapping reads proportionally to the …
usage of PLINK in the family study
written 10.5 years ago by teesnadi •

Hello, Could anybody tell me whether PLINK can be used to test association for Family based study or not? Not families but when this is …
Read orientation originated from a library was made by dUTP method
written 10.9 years ago by seta

Hi all, I've got Illumina sequencing reads that generated by the TruSeq Stranded mRNA Sample Prep Kit. Based on Trinity's guide, the first read (/1) …
Genotype of cancer genomes
written 10.9 years ago by MAPK

I have a question on whole exome sequencing of cancer. I have three different cell lines from same individual cultured separately under different treatment stages …