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can i use any rna-seq dataset for viral quasispecies estimation?

Hello,

I know there is specific method for viral quasispecies estimation called CirSeq. As compared to standard RNA-seq protocol CirSeq data contain less error rate.

Can a RNA-seq dataset generated using standard protocols be used for viral quasispecies estimation?

If yes, what should be the read depth?

Thank You.

viral quasispecies rnaseq

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