This is a test version of Biostars. For the public version, visit https://www.biostars.org.
BLESS : ERROR: Irregular quality score range 35-75

I get follows error output when used the bless to correct my Illumina paired-end reads, which I have trimmed used Trimmomatic software.

Checking input read files

ERROR: Irregular quality score range 35-75

--------------------------------------------------------------------------
MPI_ABORT was invoked on rank 0 in communicator MPI_COMM_WORLD 
with errorcode 103.

NOTE: invoking MPI_ABORT causes Open MPI to kill all MPI processes.
You may or may not see output from other processes, depending on
exactly when Open MPI kills them.
software error next-gen

Command-line used? BLESS version?

Yes, command-line, VERSION: 1.02

I meant the exact command-line you used.

bless -read1 3k_R1.rd.clean.fastq -read2 3k_R2.rd.clean.fastq -prefix ../bless/3k_R12.rd.clean -kmerlength 31

0 answers

No answers yet.

Log in to answer this question.