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Question: blast alignemnt query coverage

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Can anyone tell me the error in this command. I want to get full query coverage also with my seq. alignment and when I am using qcovs it is showing error.

blastn -db 17978_old_complete_genome -query 17978new_ol_genes.fasta -perc_identity 90 -evalue 0.0001 -qcovs -outfmt 6  > complete_genome_0.0001_oneline_output_qcovs

blast • 1.4k views

ADD COMMENT • link • updated 2.4 years ago geek_y 9.7k

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Why don't you state the error that you are getting while running the command

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Karma • 200

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The pattern of somehow receiving upvotes for trivial comments continues into your new account, Mask/snijesh. If you're encouraging your colleagues to do this, as you mentioned in your previous comments, please do not do that. If not, I direct this message to the OP sharmatina189059 and ask them to use upvotes with caution and award them to posts that contribute significantly to the subject matter at hand.

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RamRS 21k

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I am not sure what you are worried about. I did not tell anyone to upvote my comments.

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Not to debate semantics, but I'm not "worried" about anything.

You remarked on how Mask was your previous account (See: C: RNA-Seq or Exome Seq for Clinical data analysis ). Using the older account, you also said you'd asked your colleagues to up-vote your comments. (See: C: Getting error in running GATK (GenomeAnalysisTK-3.7)? ), which we observed as odd owing to them being up-votes to relatively trivial comments.

Here, the pattern of receiving up-votes to trivial comments continues, so I asked you if your colleagues were still doing this, or if this were a one-off occurrence (a possibility I addressed with the comment directed to /u/sharmatina189059 ).

I hope this clears stuff up for you.

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RamRS 21k

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I am not using the account you mentioned and I don't know who upvoted my comments here.

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Thank you for clearing that up. New users tend to upvote any response they get, so that might be the case here.

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RamRS 21k

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Just try the same command with quotes The command string needs these quotes, such that the command line looks like:

blastn -outfmt "6 qcovs" ...

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Thanks ..it works but which coloumn gives query coverage

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sharmatina189059 • 30

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why don't you upload your results.. at-least first 10 lines

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Output should be in the same order as what you specified in -outfmt 6 "sseqid qseq qcovs sseq" gives you

gi|672239066|ref|NR_125655.1|   AACTGACAGTTTTAGAAACAGGACT   2   AATTGACAGTTTTAGAAATAGAACT
gi|631253076|ref|NR_114274.1|   AACTGACAGTTTTAGAAACAGGACT   2   AATTGACAGTTTTAGAAATAGAACT
gi|636560310|ref|NR_116370.1|   AACTGACAGTTTTAGAAACAGGACT   2   AATTGACAGTTTTAGAAATAGAACT
gi|343198619|ref|NR_043083.1|   CTAAAAGTTACCCCACCGAC    2   CTAAAGGTTACCCCACCGAC
gi|343201461|ref|NR_042187.1|   AACTGACAGTTTTAGAAACAGGACT   2   AATTGACAGTTTTAGAAATAGAACT

Here qcovs is in column 3.

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genomax 68k

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