Why adding adapter first and probe later, but not the opposite?
I saw it is common to add adapter first and then use probe to grep interested fragments in lots of NGS protocol.
I am wonder why don't we do the opposite? Would't that be more efficient since that way we can only target fragments being bind with probes and don't have to waste adapters on unwanted fragments? Is there any technical reason that make the protocol the way it is? Any comments are appreciate!
• 584 views
•
link
0 answers
No answers yet.
Log in to answer this question.
Hello CY!
We believe that this post does not fit the main topic of this site.
Not bioinformatics, ask at seqanswers.com
For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.
If you disagree please tell us why in a reply below, we'll be happy to talk about it.
Cheers!