This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Transcripts read count

Hi

Which is the most correct way to calculate Transcripts read count for differential transcript expression?

I have come across using RSEM software with bam file containing transcript coordinates.

Also using script prepDE.py provided by StringTie assembler that use bam file with genomic coordinates.

Can I opt any one of these option? Or should I use any other option?

Thanks Philge Philip

rna-seq transcript read count
• 2,730 views • link
updated by andrew.j.skelton73 andrew.j.skelton73 • written by philgev2 • philgev2

1 answer

Check out Kallisto -> Sleuth or Salmon -> Wasabi -> Sleuth for modern differential transcript expression solutions.

• 0 views • link
written by andrew.j.skelton73 andrew.j.skelton73

Log in to answer this question.

More posts like this