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Calculate scaffold coverage without ambiguous (N) bases

Hi All,

I am currently trying to calculate the coverage of polar bear scaffolds in the attempt to compare between male and female coverage. I have used samtools mpileup and bedtools genomecov but I am unable to select an option which allows me to to choose only called bases in the depth calculation. So my question is whether there is any options which allows you to only choose called bases when calculating read depth/coverage.

Thanks

genome coverage

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