Fungal Metagenome Query
Hello all..
I am using UNITE database for soil fungal metagenome analysis and I have few queries.
- What is the Species Hypotheses (SH) threshold (0.5 to 3.0%) used in UNITE and if we have to change the threshold how can we change it ?
- Is there any change in annotation if we change the threshold ?
- On what basis the default (1.5%) is defined ?
- What is the optimal threshold that can be used to analyse the fungal metagenome data ?
Thanks in advance.
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Hello subodh!
It appears that your post has been cross-posted to another site: http://seqanswers.com/forums/showthread.php?p=208981
This is typically not recommended as it runs the risk of annoying people in both communities.
Sorry for that.
Actually I am badly stuck with the problem so posted in both communities to get the solution faster from the experienced persons.
Anyways, thanks for the information.