Lichee filters all variants as germline
Hello,
I'm attempting to use Lichee to construct a phylogenetic tree. However, every one of my variants are being filtered as germline.
Sample input:
chrX 46887392 G/A_JADE3 0.0 0 0 0 0 0 0 0.403846153846
Error:
**Filtered as germline:
chr8 144945137 T/G_EPPK1 0.0 0 0 0 0 0 0.202857142857 0
How I'm running it:
./lichee -build -i output.trees -n 0 -maxVAFAbsent 1 -minVAFPresent 0 -dot
Thanks so much!
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1 answer
I figured it out on my own - the maxVAFAbsent and minVAFPresent flags need to be set to values such as .005 to work.
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