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How to set the blast database of TopHat-Fusion

I am using tophat fusion. My RNA-Seq data are from human, do I need to download the other_genomic* and nt* database from blast database as suggested by the user manual of TOPHAT-Fusion? http://ccb.jhu.edu/software/tophat/fusion_tutorial.shtml Anyone who is familiar with tophat can tell me? Thanks!

blast tophat database gene fusion
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updated by Istvan Albert Istvan Albert • written by shiyang93 • shiyang93

I have the same questions. Were you able to get TopHat-Fusion working with only the human_genomic* files?

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written by kylevoyto • kylevoyto

1 answer

Since nt also contains the human genomic data it sounds like the other databases would only be needed when processing data other than human.

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written by Istvan Albert Istvan Albert

Thank you very much! By the way, there are only human_genomic.00 -08, 10 - 16, 18 -21 on the blast database, no 09 and 17. I do not know if this human_genomic* database is intact. Could you give me some advice?

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written by shiyang93 • shiyang93

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