Can Hiseq Xten used for transcriptome sequencing?

Dear all,

We want to ask if Hiseq Xten could be used for transcriptome sequencing. The illumina official said they are not designed for transcriptome seq. So any problems if using this?

Thanks a lot, Camel

hiseq platform sequencing

• 1,450 views • link

written 9.1 years ago by camelbbs

Hello camelbbs!

We believe that this post does not fit the main topic of this site.

Not bioinformatics, ask at seqanswers.com

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

• 0 views • link

written 9.1 years ago by WouterDeCoster

0 answers

No answers yet.

More posts like this

Transcriptome sequencing platform
written 4.0 years ago by umeshtanwar2 •

Hi all, I have a basic question. We are planning to perform transcriptome sequencing of leaf samples from Barley plants. We have two options, DNBSEQTM …
Map TCR sequences from bulk mRNA seq
written 4.6 years ago by chi.delta •

Dear all, I was provided with some sequencing data (FASTQ unmapped files) from an external collaborator's lab where they sorted T cells from PBMCs, isolated …
BayesHammer in SPAdes
written 5.8 years ago by Nunu •

Hello community, I'm using SPAdes to do de novo transcriptome assembly using RNA-seq (from Illumina). However, the new version SPAdes 3.14.1 has excluded the read …
de novo transcriptome assembly of Oxford Nanopore Technologies long reads
written 8.0 years ago by Alicia •

Dear all, I am currently writing a project that would involve gene expression of different non-model animals. Until now I've been working with Illumina, generating …
Please suggest an appropriate genome-guided transcriptome assembler
written 8.1 years ago by seta

Dear all, I have RNA-seq data generated by Illumina Hiseq 2000 as 100bp, PE from human, control and diseased samples. I'm looking for polymorphic simple …
IRES prediction tool
written 9.1 years ago by camelbbs

Hi all, we want to ask if there is a tool can predict the Internal ribosome entry site (IRES). We have ~200k sequences and it …
Do I need to consider strand information in searching microRNA binding sites ?
written 10.4 years ago by camelbbs

Hi all, I want to ask when we extract genomic sequences to predict the microRNA binding sites, do we need to consider the strand information …
Retrieve RNA-protein binding sites from CLIP-Seq
written 10.4 years ago by camelbbs

Hi all, We want to map the possbile RNA-protein binding (RBP) sites to our predicted transcripts. And after reading literature, we find some works extracting …
Extract sequences from local genome file by coordinates
written 10.4 years ago by camelbbs

Hi all, I want to ask if there is a bioperl module can extract sequences by coordinates (chr1:12321321-12321921) from local genome file (eg. hg19.genome.fa). We …
Should we assemble/merge R1 and R2 reads from Illumina MiSeq of fungal ITS amplicon before further …
written 11.7 years ago by sentausa

Dear all, I'm new in fungal ITS (internal transcribed spacer) metabarcoding analysis, so please ask if you don't understand my question. Our lab received Illumina …