MA plot from Differential Expression between One Sample vs another

Question

[Ron]

Hi all,

Is it possible to get MAplots when you only have foldchange and Expression values of the two samples of comparison(one vs one) ? I don't have replicates.So i only have FPKMS from 2 samples and their log2FoldChange.I know I don't have statistical significance but I still would like to have MA plots from log2Foldchange.

I have only seen MAplots from DESEQ2 results or edgeR results.

Any suggestions?

thanks,

Ron

[ATpoint]

Well, if you have your FPKMs then you can simply plot it:

M = log2(FPKM1 / FPKM2)
A = 0.5*log2(FPKM1 * FPKM2)

require(LSD)
heatscatter(x = A, y = M)