Hello!
I need to assemble a worm genome (Illumina Hiseq) using as reference a draft genome of the same species. What tool would be best for working on a 32 GB RAM workstation? And do I need any special parameters since the reference itself is a draft genome?
Thanks in advance.
1 answer
You can have a look at the following on github. I used chromosomer with some success. I would first attempt an assembly vs C. elegans or another finished genome though. To be clear, with these programs you first need to do a de novo assembly, eg. with Abyss, soap-denovo etc
fenderglass / Ragout
Chromosome assembly using multiple references
gtamazian / chromosomer
A reference-assisted assembly tool for producing draft chromosome sequences.
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