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Bioinformatics tools for genetic diversity

Guys, i want to analyse some CP gene sequences of some plant RNA viruses by HyPhy package (SLAC, FEL, REL) and also dN/dS. but i am stuck, plz help

Sample article (see materials and methods)

doi:10.1007/s00705-015-2729-z

i want to do this kind of analysis as mentioned in this paper

rna-seq

but i am stuck, plz help

So, what's wrong?

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