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species abundacy calculation

Hi all,

I'm looking for ways and softwares to represent population diversity from fastq (for exemple x% E. coli, y% human, z% P. aeruginosa reads,...)

I intend to do a local blast on different databases then used GRAMMy

Do you guys have some recommendations, comments about this approach? Is it better to start with a with de novo approach before blast? How should I deal with my reads before blast?

Thanks!

blast grammy species abundancy

What type of data are your fastq?

fastq were obtained by DNA sequencing of biological sample using Proton

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