density of reads that mapped to the specific gene
I have RNA-seq data and want to get the density of reads that mapped to the specific tRNA. do you know how to do that?
rna-seq
• 1,770 views
•
link
updated
by
Biostar
•
written
by
alirezamomeni707 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
RNA-seq analysis
written by sk_24 •I am analyzing RNA-seq data and I used HiSat2 for alignment of paired-end files. After getting the bam files (sorted by name for Htseq and …
-
Number of transcript reads with given mutation
written by misterieHi, I have a list with a specific mutations localised on one gene. I have 100 BAM RNA-seq samples, mapped to the reference genome. Let's …
-
Process RNA-Seq Data with specific sequences / not whole reference genome
written by Heinrich_M •I have specific sequences that I should quantify in RNA-Seq data. First, I blast the sequences against the reference genome. Now these data have to …
-
counting the reads mapped only to the coding sequence
written by alirezamomeni707 •I have aligned the RNAseq data to the transcriptome and have .bam files. now I want to count the reads that map to the coding …
-
alignment to a special part
written by alirezamomeni707 •I have a RNA-seq data and want to get the proportion of reads that map to a window of `100pb` between `5'UTR` and `CDS` (`50 …
-
using DREME and .broadpeak file
written by alirezamomeni707 •I have chip-seq data and used MACS2 to find peaks (genomic regions enriched for methylation). now I want to find the motifs. I found a …
-
how to get the coverage for the entire interval
written by alirezamomeni707 •I have aligned my `RNA-seq` data and trying to get the coverage of 72 `nt` sequence using `bedtools`. I also have the coordinate of this …
-
counting the reads mapped to specific coordinate in the genome
written by alirezamomeni707 •I have RNA-seq data and aligned them to the genome, so I also have bam files. I also have the sequence of tRNA genes in …
-
alignment of sequencing data to the tRNA transcriptome
written by alirezamomeni707 •I have ribosome sequencing data and want to align it to the tRNA (not mRNA transcriptome). I have filtered out rRNA, non-coding RNAs and adapter. …
-
Isolating reads from specific region from bam file
written by SaraI have RNA-seq data and would like to get the reads from specific region for example -/+ 50 reads before and after stop site. do …
Density? Do you mean counting the reads overlapping a gene?
yes. and then I would make a density plot.
For counting reads you can use tools such as featureCounts and htseq-count.