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Identifying sample from FASTQ file

I have a very Old Fastq file that looks as-

@ILLUMINA-DB1410_0001:1:1:1092:14610#0/1
CTAAATAAGNCCTTTCCCCACCTGTTTGATTCTGTTTCCT
+ILLUMINA-DB1410_0001:1:1:1092:14610#0/1
bbbbbbbbbBbbbbbbbbbbbaabbbbbbbbbbbbbbbbb
@ILLUMINA-DB1410_0001:1:1:1092:7332#0/1
CACAGTCTCNTGGGGGATGAATGACAAGTGAGCAGAATGT
+ILLUMINA-DB1410_0001:1:1:1092:7332#0/1
bbbbbbbbbB^]^]_XUZ[WGOOOO`^^]^aaaaa_`aWa

Now I want to find out the sample as I have bar codes like s_5_CGTACG_sequence.txt s_5_GAGTGG_sequence.txt s_5_ACTGAT_sequence.txt

Is there any way that I can find out this FASTq file is associated with any of above three barcodes.

I read about FAStQ format but could not find any solution

Thanks

fastq
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updated by GenoMax GenoMax • written by joerodger2017 • joerodger2017

1 answer

I am afraid you are correct that you can't tell what sample this is by looking at this data. As you noted there are no barcodes in the fastq headers (unless they were replaced by #0, which again won't help unless you have a key for that code).

If you had other independent data (e.g. if the three samples were from three distinct organisms/genomes or had specific SNP's which could be identified in the data by using a known reference) then you may be able to make some progress.

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written by GenoMax GenoMax

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