Entering edit mode
7.1 years ago
jingjin2203
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60
Hi All, I am working on a in silico genome comparison project. I've done the in silico enzyme digestion with my 16 different genomes (they belong to the same species, I will be using one of them as reference genome) and got the DNA fragments in desired size in FASTA file.
I had several questions that stop me moving forward, hope you guys can help me out. My specific questions are: 1) What tools can I use for calling SNPs? 2) Does SNPs calling require FASTQ file? If so, is it possible to change FASTA file to FASTQ file by faking quality scores? And which tools can I use to do that?
Thanks for your kind attention! I will really appreciate your help!
Best, Jing
