Identify kinase that phosphorylates/dephosphorylates a specific protein

Question

[m.koohi.m]

Dear friends,

I am wondering if there is any bioinformatic approach or database to find the kinase that phosphorylates/dephosphorylates a specific protein?

I have a protein of E.Coli and want to find which kinase potentially can phosphorylates/dephosphorylates it.

Thank you,

[Lars Juhl Jensen]

If all you have is a protein, trying to predict upstream kinases would be an exercise in futility. At the very least, you would have to know the specific phosphorylation site for which you want predictions. However, the databases and tools that I know of all focus on eukaryotic phosphorylation and thus would be useless for E. coli.

[m.koohi.m]

Thanks Lars, Yes, the problem is all of the databases are for eukaryotic as I checked. I actually know the phosphorylation site. You think it can helpful to find at least the type of kinase?

[Jean-Karim Heriche]

If I remember correctly, bacterial protein kinases tend to not have strong substrate specificity in comparison to eukaryotic kinases.