RNA-Seq/Statistical Analysis Design Question
Hi everyone, I just have a basic design question. For my analysis I want to compare two groups of patients (CKD vs Non-CKD). I have 1 sample from each of the 5 Non-CKD patients, 1 sample from each of 4 CKD patients, and 2 samples from 1 CKD patient. Would it be alright to use the 2 samples from the same patient at the same time or would it be better to just include 1 sample? Thank you very much!
Edit: Wording
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What Is the difference between those two samples? are they different conditions ?
No, they are from the same condition and would be in the same group for analysis.
I guess the most appropriate would be to merge both datasets of the same condition/sample/individual.