Hi, I'm trying to conduct parametric linkage analysis using SNP data and merlin. I'm using the basic command used in the merlin tutorial:
merlin -d test.dat -p test.ped -m test.map --model parametric.model --step 3 --bits 26 --megabytes:2048
and am receiving LOD scores of 0 for every single marker. I have also tried the basic command for non-parametric linkage and an error informing me this was an uninformative family was received.
I cannot see any discrepancies between my input files (.ped, .dat, .map) and the examples supplied by merlin. I have tried applying mock affection statuses to see if this is the issue, to no avail. The only issue I have found is that the pedstats summary of my pedigree reads:
Generations
Average: 5.00 (5 to 5)
Distribution: 5 (100.0%), 0 (0.0%) and 1 (0.0%)
While this correctly finds 5 generations in my pedigree, the distribution is wrong. This leads me to believe the ped file is the issue, however I have used it successfully in other programs, including paramlink in R.
I would attach examples of my files but am new here and cannot see a way to do so. Any ideas as to what might be going on or what to try would be most appreciated.
1 answer
The no informative families found error is basically happens when there is no complete pedigree information present, make sure your ped file consist complete pedigree information(complete trios), incase if any member is absent make a dummy profile for them.
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Hi,
Have you by chance had any luck in this? I am also seeing the exact same problem. Thank you very much in advance!