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Which parameters to change in Tophat default settings for homosapiens cancer data?

I am working with RNA-Sequencing. I have to compare my data with microarray data. i did Tophat, cufflinks and cuffdiff with default parameters so i am not able to achieve the perfect result. my question is do i have to add any optional parameters in my command. if is there please help me..

rna-seq next-gen software error

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