How to design matrix for LIMMA between control and stress conditions to calculate logFC and p-value.
I am new to data sciences and R programming. I have normalized data files separate for control and diseased conditions. It has Gene ID as rows and expression values as columns. How do I design a matrix for LIMMA to calculate logFC, P-Value and adjusted P-Value between control and stress conditions. And what input data file should I provide as input for the lmFit function so that it compares between control and stress data sets.
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asnanizami •
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limma has an excellent user guide, please read through it and reply if your question isn't answered.