miRNA sequencing by Illumina platform
Hi all !! I have sent porcine PBMC samples for miRNA sequencing. After passing the quality check (RIN value = 6.9) I was informed that the sequencing was failed (number of reads were less ranging from thousands to 7 millions for 3 different samples) . Is there any possibility of failing sequencing after passing QC? What will be the reasons for getting less sequencing reads?
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lrp.sailo •
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It sounds like you need to work with your sequencing facility and/or illumina tech support to understand what the problem is in this case. You may want to post this over at http://seqanswers.com.