Housekeeper Genes and Real Time PCR · Biostar

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Housekeeper Genes and Real Time PCR

I am new to RT-PCR, Real-Time PCR, as well as working with housekeeper genes. I analyze mice samples to determine if they have Lactate Dehydrogenase- elevating virus. I was given the task to find a house keeper gene that could help us ensure a negative result is truly a negative. I have searched literature and have found nothing published where this have been done. Can anyone led me in the direction of finding how to determine the best housekeeper genes and their primer sequences to accomplish the job I am trying to do? Thanks to anyone who can assist.

gene pcr housekeeper gene

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written 9.6 years ago by Maria.sewell •

Try to search NCBI: www.ncbi.nlm.nih.gov

and use the following key-words:

mouse AND RT-PCR AND lactate dehydrogenase elevating virus AND house keeping gene

I've found 12 articles, some may be helpful to you.

• 0 views • link

written 9.6 years ago by natasha.sernova

Perhaps this post accompanying a recent article is interesting for you.

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written 9.6 years ago by WouterDeCoster

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