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choose the "best"mutation from a list

I have a list of mutations (around 400) from COMIC database. I have given a score to each mutation from different tools e.g by position, conservation, blosum, patogenecity...for each mutation position. My goal is to find a "easy" mathematical model or a model to choose from this list the "best" mutation (around 10 mutants) to take to cell culture lab. Could you help what model I can use? I was thinking something like nX+nY+nZ. X, Y, Z are the score from different tools I got and n would be 1/3

sequence blast assembly
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written by fifivater • fifivater

1 answer

What does it mean for a mutation to be the best? Do you mean "the most likely pathogenic"?

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written by WouterDeCoster WouterDeCoster

Yes, I mean the most pathogenic mutant. So I have calculated score for each mutants (~300) by different tools e.g. Blosum, PON-P2, ConSurf. Also i have given a score by position. The Scores from each tool has been normalized betwen 0-1. So my plan is to find a simple mathematical model to choose from this list ~10-20 pathogenic mutant to take to the next level. I need somehow take consider scores from each tool, and find what final score it will give me.

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written by fifivater • fifivater

Thank you very much for the hints, I will get familiar myself with those tools Thanks :)

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written by fifivater • fifivater

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