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Samtools sam-> Bam

Hello,

I have a list of sam files that were created using HISAT2. I need to convert all of them into bam files for downstream analysis and I do so using the following (so it runs on all sam files in the directory)

samtools view -@ 7 -b -S -o *.sam > *.bam

the operation runs but nothing happens in the output files (I can't even see them). Ideally I would want a bam file being in the output titled as the sam file that was used for input.

Help..?

Thanks!!

rnaseq sam bam samtools
• 5,326 views • link
updated by Devon Ryan Devon Ryan • written by V V 
for f in *.sam; do filename="${f%.*}"; samtools view -@ 7 -bS $f > $filename".bam"; done
• 0 views • link
written by Medhat Medhat

1 answer

You're not going to do this with shell globbing (i.e., *.sam and *.bam). You're either going to write a little for loop in bash or something like python or snakemake. I find snakemake quite simple to use, in which case you'd do:

import glob
files = glob.glob("*.sam")
targets = ["{}.bam".format(x[:-4]) for x in files]

rule all:
    input: targets

rule convert:
    input "{sample}.sam"
    output: "{sample}.bam"
    threads: 7
    shell: "samtools view -@ {threads} -b -o {output} {input}"

I've taken the liberty of fixing the samtools command.

• 0 views • link
written by Devon Ryan Devon Ryan

Devon is right. just to be a little bit more explicit, the quick bash alternative to generate .bam files from .sam files would be

for file in *.sam; do samtools view -bS $file > ${file/.sam/.bam}; done
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written by Jorge Amigo Jorge Amigo

Thanks Devon & Jorge!! Jorge's approach was more straightforward as I'm new to ubuntu. I tried Devon's but it kept running into different issues and trying to run a "Yagi=Uda antenna analysis program" for some reason. Probably a mistake I did on my end even though I only copied and pasted the command.

Thanks again!

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written by V V

Thanks for providing the bash version, my bash-fu has been deteriorating since snakemake was introduced :P

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written by Devon Ryan Devon Ryan

Hi Devon,

When I actually paste your code in the first thing that happens is that my pointer turns into a target looking thing and I have to click somewhere (and then everything crashes) what is it that the import glob actually does??

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written by V V

This is python/snakemake code, so you would need to write this to a file. import glob imports the glob python module, which allows things like "give me all files ending in '.sam' in the current directory" (glob.glob("*.sam")).

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written by Devon Ryan Devon Ryan

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