best way to find downstream effects of insertion

Question

[badribio]

what is the best way to find if an insertion on a particular exon of a gene results in complete frameshift or any interference in splicing in other exons due to insertion in the reading frame?

is Ensemble VEP a right approach?

[WouterDeCoster]

The best way is probably cDNA sequencing, qPCR and western blot...

Answer 1

[Medhat]

Yest It is and here is another tool that also do a good job
SnpEff is a variant annotation and effect prediction tool. It annotates and predicts the effects of variants on genes (such as amino acid changes).