This is a simple formula used to calculate the temperature: Tm = 2(A+T) + 4(G+C) ℃
I'm curious about the Nearest-neighbor method.
I get the deltaH and deltaS but when I put this 2 parameter in the formula I got a very small number than the correct answer. Can someone teach me how to count?
wiki: https://en.wikipedia.org/wiki/Nucleic_acid_thermodynamics
This is my primer : GCTAGTTCAACGTCAAGTGTG
Enthalpy : -167 cal/mol
Entropy : -455.9 cal/mol-K
I use this formula
Tm = delta H / (delta S + 1.99 x ( ln(c/4)) - 273.15 = 87.14 C
Tm = (-167 ) / ( -455.9 + 1.99 x ln(c/4)) - 273.15 = -272.8
87.14(correct)
-272.15(my answer)
where c is probe concentration. In this case 0.1
Can someone teach me how to count? Thanks!