Thank you very much. I just started reviewing, it's awesome.
Hi everyone,
I am bioinformatician and I worked on "Mathematical Modeling and Simulation" of signaling pathways. Now, I want to work on Microarray data. I am new in this field, and I didn't know more about microarray data. How we generate (criteria) the excel file, which includes (Gene name,Probe.., Intensity, log ratio, etc) from our dataset. If the data is already normalized then what will be the next step? Is there any good tutorial for beginner, which guides step by step from data selection to analysis. I need the valuable suggestions from all the experts in this field.
Thank you
2 answers
If you are beginner in microarrays, please follow the limma user's guide.
http://www.bioconductor.org/packages/devel/bioc/vignettes/limma/inst/doc/usersguide.pdf
Have a look at following links:
Using Bioconductor for Microarray Analysis
https://www.bioconductor.org/help/workflows/arrays/
Analysing Microarray Data In Bioconductor
Analysing Microarray Data In Bioconductor
Microarray analysis exercises
http://jura.wi.mit.edu/bio/education/bioinfo2007/arrays/array_exercises_1R.html
Analyze your own microarray data in R/Bioconductor
http://wiki.bits.vib.be/index.php/Analyze_your_own_microarray_data_in_R/Bioconductor
Analysing microarray data in BioConductor
http://bioinformatics.knowledgeblog.org/2011/06/20/analysing-microarray-data-in-bioconductor/
Microarray Data Analysis
https://bioinformatics.ca/workshops/2012/microarray-data-analysis
Thank you for valuable share, I am going to follow these links.
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I would highly recommend this tutorial to get you started.
Analysing Microarray Data In Bioconductor