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Cufflinks processed loci numbers

Hi, I am quite new to rna-seq analysis work flow. After TopHat alignment, I ran cufflinks, cuffmerge, cuffquant, and cuffdiff, respectively. The number of processed loci I obtained from each cuff* is slightly different. Is there anything wrong with that? or it is possible to have different numbers?

Thank you for your helps

rna-seq

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