Novel genes identify
Hi,
Can someone explain to me how can i identify the novel genes in my set of gene of non-model organism ? is the novel gene ,the sequences which does not match similarity with other organism when functional annotation run ?? So all sequences with no BLAST results are novels??
Thanks
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Genes annotated as hypothetical proteins usually do not have close homologs and hence novel?
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That is pretty much the basics of it. First you would have to do some sort of gene prediction on your non-model organism genome - but I guess you have done that as you say you have a gene set. Then of those genes, the "novel" ones are those that cannot be mapped with confidence to existing entries in any public sequence database - so certainly those with no BLAST hits., but even if a gene had a BLAST hit, it may be pretty a poor match.