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CRISPER genome wide screen

I have CRISPER seq fastq files and want to find out level of Sg RNA enrichment in mouse genome. Is there any standard work flow for such analysis. Any pointer will be useful

Thanks

geomw wide screen
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updated by morovatunc morovatunc • written by rob.costa1234 rob.costa1234

1 answer

Hi,

You can give a try to MaGeCK. Especially with their most recent update, they have implemented a pipeline that handles very raw data such fastq. Also they are quite responsive in their forum.

https://sourceforge.net/p/mageck/wiki/Home/

Hope this helps,

Best,

T.

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written by morovatunc morovatunc

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