VelvetOptimiser getting an unknown option error
I'm trying to run VelvetOptimiser, and I keep getting the following error:
VelvetOptimiser.pl Version 2.2.5
Number of CPUs available: 32
Current free RAM: 122.174GB
Velvet OMP compiler setting: 1
Unknown option: fasta -shortpaired pairedreads_paired.fasta -fasta -short singlereads_singletons.fasta
This is my command:
perl ./programs/VelvetOptimiser-2.2.5/VelvetOptimiser.pl –s 51 –e 71 –o'-ins_length 350' -f '-fasta -shortPaired PairedReads_paired.fasta -fasta -short SingleReads_singletons.fasta'
Does anyone know what is happening here? For some reason it's getting rid of the "-" in front of fasta in the velveth options, but I can't seem to get it to work at all.
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Pryce Michener •
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Are you sure you have not copied and pasted the command from web or any other documents, as it sounds like a typical copy-paste command issue. Make sure that you type the entire command on the terminal and check if the error disappears!