Hi there , recently I want to use birte package (http://bioconductor.org/packages/release/bioc/html/birte.html) to analysis my own data , but with no luck I suffered some errors such as below:

Here is the R command:

mRNA_result = birteLimma(dat.mRNA=mydat, limmamRNA=limmamRNA, affinities=affinities2,niter=500, nburnin=5000, thin=1)

And the error shows:

Automatic assignement of #replicates with design matrix (mRNA data). Formatting regulator-target network -> checking overlap between network and measurements. --> biRte tries to explain mRNA log fold changes TF Calculating potential swaps for regulator type TF miRNA Calculating potential swaps for regulator type miRNA Error in fitdistr(1/mRNA.Sigma^2, "gamma") : 'x' must be a non-empty numeric vector

anyone can help ? Thank you a lot