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How to use multifasta file in EMBOSS plotORF ?

Dear Friends, Hi

I want to use plotorf to visualize the theoretical ORF in a bunch of transcript sequences, but it seems that the program is running just for the first sequence of the FASTA file.

would you please help me about running plotORF on all my transcripts in just one command ?

~ Best

software error sequence emboss orf
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updated by Sej Modha Sej Modha • written by Farbod Farbod

1 answer

Plotorf only works with single nucleotide sequence.

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written by Sej Modha Sej Modha

Dear Sej Modha, hi and thanks,

So, what must we do when we have 200 nucleotide sequences?

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written by Farbod Farbod

I am not aware of any other tools that can do this, worth checking bioconductor packages in R or write a loop to run plotorf for each sequence individually.

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written by Sej Modha Sej Modha

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