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Assembly out of mapping reads to a reference genome

Hi, It seems nobody get assembly out of mapping reads to reference genome. Is there a reason why this approach is not good?

Thank you in advance.

Mic

assembly

When mapping reads to a reference genome, what you would have is a consensus, not an assembly. This would be a reference with variants introduced from your mapping data. If you mean to say that assembly of the reads which map to a genome is fragmented, it is due to the uneven coverage, genome complexity and repeats. There are approaches to make local assemblies out of mapped reads, which work to an extent.

Could please provide examples how to make local assemblies out of mapped reads?

You can try ABRA for local assemblies.

You can do both reference-guided as well as denovo genome assembly.

Could you please provide examples for reference-guided assembly?

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