Help with locating start site of a DNA fragment.
Hello!
I need some assistance with the following questions:
1. Using the sequence below - provide the sequence in fasta format of the CDS encoded by this DNA fragment. How did you choose the start site? Detail your complete analysis that led to your choice of Start site.
CTAAATGTTAAAAGGTGCCCCTCAACAAAAAAGACACACAGGGGAAAGGC
GTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCC
GACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGG
GTGGCGCAATTGGTACTGGTCTGTTTCTTGGCATTGGCCCGGCGATTCAG
ATGGCGGGTCCGGCTGTATTGCTGGGCTACGGCGTCGCCGGGATCATCGC
TTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAGGAGCCGGTAT
CCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGC
TTCCTCTCTGGCTGGAACTACTGGGTAATGTTCGTGCTGGTGGGAATGGC
AGAGCTGACCGCTGCGGGCATCTATATGCAGTACTGGTTCCCGGATGTTC
CAACGTGGATTTGGGCTGCCGCCTTCTTTATTATCATCAACGCCGTTAAC
CTGGTGAACGTGCGCTTATATGGCGAAACCGAGTTCTGGTTTGCGTTGAT
TAAAGTGCTGGCAATCATCGGTATGATCGGCTTTGGCCTGTGGCTGCTGT
TTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTAC
GGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTGGCGGT
AATTATGTTCTCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTG
AAGCGCGCGATCCGGAAAAAAGCATTCCAAAAGCGGTAAATCAGGTGGTG
TATCGCATCCTGCTGTTTTACATCGGTTCACTGGTGGTTTTACTGGCGCT
CTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTTGTGATGATTT
TCCATAATCTCGACAGCAACGTGGTAGCTTCTGCGCTGAACTTCGTCATT
CTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCG
CATGCTGTTTGGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTC
GCGTCAGCCGTCGCGGTGTGCCGATTAACTCGCTGATGCTTTCCGGAGCG
ATCACTTCGCTGGTGGTGTTAATCAACTATCTGCTGCCGCAAAAAGCGTT
TGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTGAACTGGATTA
TGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGG
CGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTG
CATTGCCTTCCTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATA
TGCGCTTGTCAGCGATCCTGCTGCCGGTGTGGATTGTATTCCTGTTTATG
GCATTTAAAACGCTGCGTCGGAAATAAGGCATTCACGCTACATCCGACAA
AACGA
TGTCAACCA
TCCGAAACCGCTC
2. Where do you think the protein you identified in question 1 and the protein 2 given below are located in the cell? Present the bioinformatic evidence that led you to these conclusions. What do you think is the sequence of the mature protein 2? Paste it in Fasta format.
Protein 2
MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITPVGVSSYSDYPPQAQKI
EQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGIKVMWVDATSIEQIANA
LRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFLQFGINPPFTSGKESIQN
QVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGPDQIPKIKQYWGEQLKIPVIP
LTSDWFERASPRIILAAQQLCNALSQVD
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Thank you so much for your help!! :)
This looks a lot like a homework assignment, which you should do on your own*. You are not helping yourself by just outsourcing the task to strangers on the internet. I and many others here already have our degree.
*Unless you already wrote a lot of code and got stuck, show effort and you are more likely to get help